Additionally, we have performed rescue experiment in KATO III and MKN45 GRK3 OE cells by knock down YAP1 using LentiCRISPR/CAS9 and found that GRK3 OE significantly increased tumor cell invasion and tumor sphere formation in both KATO III and MKN45 cells, while KD YAP1 in GRK3 OE cells dramatically reduced the capability of invasion and tumor sphere formation induced by GRK3 OR in both KATO III and MKN45 cell lines (Supplementary Figs. 5A-5D). This evidence concerns the gene GRK3 and neoplasm.