To test whether the anti-PD-1 scFv can restore the production of IL-2 and IFN-γ by Jurkat T cells, the stimulated Jurkat cells were co-cultured with A549 and HepG2 tumor cells lines, which were pre-treated with IFN-γ to express PD-L1 receptor. The gene discussed is IFNG; the disease is neoplasm.