In addition, enrichment analysis showed that TMEM220-AS1 is involved in the cell cycle, DNA replication pathways, p53 signaling pathway, etc., and F11-AS1 is involved in non-alcoholic fatty liver disease, fatty acid degradation pathways, etc. The cell cycle is a complex process that is regulated by a variety of proteins at multiple levels, and the cell cycle pathway plays a crucial role in tumorigenesis31. Here, TMEM220 is linked to metabolic dysfunction-associated steatotic liver disease.