▪Gas plasma elevated tumour cell death in microtissues by activation of caspase 3 and reduced microsatellite growth in a 3D model system▪Reduced migratory activity of Tumour cells in microtissues▪Gas plasma exposure modified the release of several inflammatory agents in the TME, such as IL‐6, IL‐8, IL‐18, IL‐33, IL‐17A, IFN‐α2 and MCP1. This evidence concerns the gene IL33 and neoplasm.