▪Gas plasma elevated tumour cell death in microtissues by activation of caspase 3 and reduced microsatellite growth in a 3D model system▪Reduced migratory activity of Tumour cells in microtissues▪Gas plasma exposure modified the release of several inflammatory agents in the TME, such as IL‐6, IL‐8, IL‐18, IL‐33, IL‐17A, IFN‐α2 and MCP1. The gene discussed is IL18; the disease is neoplasm.