Among others, we selected to alter amino acid residues that interact with other r-proteins and rRNA domains of the 60S ribosomal subunit, and residues found mutated in human r-protein rpL35A/eL33 related with DBA and different tumors, to try and determine in S. cerevisiae the functional consequences of substitutions associated with DBA and cancer. The gene discussed is RPL35A; the disease is cancer.