Bovine trypsin also acts as a serine protease; thus, it is speculated that the reduction of EPS in the biofilm of periodontitis-related microorganisms established in this experiment may be related to the hydrolysis of cell surface proteins; (2) Bovine trypsin can hydrolyze bacterial extracellular proteins, which are an important part of EPS. The gene discussed is PROS1; the disease is periodontitis.