Genetically engineered chimeric antigen receptors (CARs) T lymphatic cell and natural T lymphatic cell that extracted from human T cells enriched from peripheral blood mononuclear cells have been obtained, the CAR-T cells could recognize GPC3 expressed on the surface of hepatocellular carcinoma cells, enhancing targeting ability, and the CAR T cells could eliminate cancer cells by single-chain variable region (ScFv) on the cell membranes of CAR-T cells, in a non-major histocompatibility complex-restricted way (Ma et al., 2020). The gene discussed is GPC3; the disease is cancer.