The results of this study showed that the apoptosis rate of hypothalamic neurons and pituitary tissue cells in the WD group was significantly increased compared with the normal control group, and the expression levels of pro-apoptotic proteins Bax and Caspase-3 were significantly increased compared with the NC group, while the expression level of the anti-apoptotic protein Bcl-2 was significantly decreased. Here, CASP3 is linked to Wilson disease.