Further analysis of markers of glioma stem cells (GSCs) was performed, including surface markers, spectral markers, and transcription factors (TFs), where among cell surface markers, CD24 was highest in NPC-like cells, CD133 in OPC-like cells, EGFR in AC-like cells, and CD44 in MES-like cells, and among TFs and spectral markers, NES showed a significant bias toward AC-like cells (14). This evidence concerns the gene CD44 and glioma.