As shown in Figure 2, compared to the construct with only B domain deleted FV versus FV constructs with the B domain deletion and replacement by the SQ linker or furin cleavage, the FVa plasmid with the furin cleavage domain showed a slight tendency to induce a better hemostasis improvement with a shorter aPTT in both hemophilia A and B mice. The gene discussed is FURIN; the disease is hemophilia A.