It is obvious that the mutation rates of these 16 genes in HCC tissues are modest; even HUWE1, which has the highest mutation rate, is just 2% (Figure 3B), and only TOMM22 and HUWE1 show an indigenous co-mutation connection, implying that either of the two mutations will cause the other (Figure 3C). Here, HUWE1 is linked to hepatocellular carcinoma.