MUC5AC and cystic fibrosis: In order to assess the influence of mucin concentration, measurement temperature, and evaporation control we first studied BSM macrorheology at different concentrations (2% and 10% solids to mimic healthy and CF mucus, respectively) and measurement conditions (25°C versus 37°C, with versus without a solvent trap) by dynamic oscillatory shear measurements consisting of an amplitude- and a strain-controlled frequency sweep using a cone-plate geometry.