To assess whether the presence of two bands in the each of the ALT and CAN samples might be due to enrichment of complexes containing both HEBAlt and HEBCan, we performed co-IPs in HEK293T cells with myc-tagged and HA-tagged HEBAlt, HEBCan, E2A, and MyoD (Figure 3G). Here, MYOD1 is linked to Crouzon syndrome-acanthosis nigricans syndrome.