The anti-leukemia cytotoxicity in vivo was validated through the human AML xenograft mouse model, in which 1 × 106 U937-EGFP-Luci leukemia cells were intravenously injected into NSG mice, followed by 5.0 × 106 Non-CAR T cells, MOCK-CAR T cells, and GRP78-CAR T cells injection at day 6 (Figure 2A). The gene discussed is HSPA5; the disease is leukemia.