WEE1 and insomnia measurement: Subsequently, 5-HT to a G protein-coupled receptor, which is catalyzed by ADCY5 to convert ATP to cAMP and then directly regulate the downstream pathway, activating the core gene protein kinase PKA, which activates the Serotonergic synapse signaling pathway, resulting in increased 5-HT and GABA expression, thus improving insomnia symptoms and relieving anxiety, see Figure 8 for the mechanism diagram.