GPT and metabolic dysfunction-associated steatohepatitis: In this study, we successfully built a NASH model by feeding mice with an MCD diet for 6 weeks, as evidenced by hepatic lipid deposition (increased hepatic TC, TG, and liver histological observations), liver function damage (elevated serum ALT and AST levels), and oxidative stress (increased hepatic MDA and decreased GSH levels) in MCD diet-fed mice.