Furthermore, in DDS-induced colitis in male C57BL/6 mice, BS was able to reduce the levels of TNF, IL-6, and IL-1 in intestinal tissue, indicating that β-sitosterol administration significantly reduced inflammatory damage to colonic tissues, including colon edema, crypt distortion, goblet cell loss, and mononuclear cell infiltration. This evidence concerns the gene IL6 and colitis.