To define the impact of POU5F1B activation, we first analyzed RNA-seq data from GFP- and POU5F1B-overexpressing HT29 and SW480 cells, from control and POU5F1B-knockdown LS1034 cells, and from stages I, II, and III SYSCOL CRC primary tumors (Supplementary Fig. 5a, b). This evidence concerns the gene POU5F1B and colorectal carcinoma.