Herein, we demonstrate that silencing PRMT5, MEP50, WDR5 or treatment with small-molecule OICR-9429, an inhibitor of WDR5 interaction with the H3 tail, significantly reduced the levels of H3R2me1 and H3R2me2s on the ANGPT2 promoter and decrease the mRNA levels of ANGPT2 in MYBL1-overexpression HCC cells (Fig. 6D and Supplementary Fig. S4A). This evidence concerns the gene WDR77 and hepatocellular carcinoma.