ANGPT2 and hepatocellular carcinoma: Luciferase reporter assay was performed and showed that overexpression of MYBL1 promoted, while silencing MYBL1 repressed, the luciferase activity of the ANGPT2 promoter in a dose-dependent manner in HCC cells but shown no effect on the luciferase activities of the promoter with a deleted MYBL1-binding site (Fig. 4D and Supplementary Fig. S2C).