PKM and Alzheimer disease: Nuclear p-PKM2 phosphorylates threonine 11 on histone 3 (H3T11-P), and indeed, immunocytochemistry showed bright H3T11-P puncta and a 1.31-fold increased H3T11-P signal in AD iNs, validating an increased protein kinase activity of PKM2 in the nucleus of AD iNs (Figures 5D and 5E).