These two modules were also associated with multiple signaling pathways linked with cancer, such as PI3K−Akt and PI3K−Akt−mTOR signaling pathway (Figure 4D, bottom).[70] Regulatory network analysis of myCAFs with GENIE3 revealed 1,242 regulatory pairs (Table S9, Supporting Information, weight > 0.05).[110] Among them, 11 transcription factors (TFs) targeting more than 30 genes (Figure 4E), which included ESR1, GLIS3, KLF5, MECOM, PRRX1, RORA, RUNX1, SOX5, TCF4, TFCP2L1,and TP63. This evidence concerns the gene ESR1 and cancer.