Compared to EMPA + CON rats, EMPA + PCOS rats had lower citrate synthase activity (32 ± 5 vs 61 ± 8 nmol/min/mg protein, P < 0.05) and Complex IV activity (210 ± 24 vs 326 ± 36 nmol e−/min/mg protein, P < 0.05). This evidence concerns the gene CS and polycystic ovary syndrome.