To examine the epigenetic correlation between infiltration of TAMs and advancement of HCC, Smart-seq based scRNA-seq method was applied to study tumor-derived CD45+ cells and four immune-relevant sites (adjacent liver, blood, ascites and hepatic LNs) of six treatment-naive liver cancer donors (data from GSE140228, Supplementary Fig. S1) [9]. This evidence concerns the gene PTPRC and hepatocellular carcinoma.