As shown in a representative case in Fig. 1E, F and Additional file 1: Fig. S1B, the proportion of CD4+ CD25high CD127low/neg Tregs was significantly increased in lymphoma tissues from DLBCL and presented low CD45RA expression, indicating that more cells were the CD4+ CD25high CD127low/neg CD45RA− effector subsets of Tregs. The gene discussed is CD4; the disease is diffuse large B-cell lymphoma.