We also found that exogenous supplementation of Fe2+ could partially reverse the inhibitory effect on Wnt/β-catenin signaling and CRC progression caused by STEAP3-AS1 knockdown, indicating the pivotal role of STEAP3-AS1/STEAP3-mediated Fe2+ generation in activating Wnt and promoting CRC progression. Here, STEAP3 is linked to colorectal carcinoma.