ITGAM and Splenomegaly: Detailed flow cytometric analysis of the immune cells present in the spleens of tumour-bearing vehicle- or PI-3065-treated animals revealed that splenomegaly was driven by a 40-fold expansion of PMN-MDSCs (CD11b+ Ly6G+ Ly6Clo) in vehicle-treated tumour-bearing animals, and to a lesser extent by expansion of M-MDSCs (CD11b+ Ly6G−Ly6Chi,, 30-fold expansion) (Fig. 1c).