Tumours were analysed by flow cytometry, one day after intra-tumoural poly(I:C), which showed YFP-positive cells were all CD45+, CD11b+, MHC-II−, F4/80−, CD11c− with the majority expressing Ly6C, consistent with the single cell RNAseq results (Fig. 5i). This evidence concerns the gene ITGAM and neoplasm.