We used this reference matrix to perform a deconvolution analysis on our bulk RNAseq data, which demonstrated that genes associated with IFNβ signalling, but not other cytokines, followed the on/fast-off IFN pattern in responders in both AB1 and Renca tumour models, suggesting IFNβ rather than IFNγ was responsible for these observed dynamics (Fig. 4c, d and Supplementary Fig. 6a, b). This evidence concerns the gene IFNA1 and neoplasm.