In addition, western blot analysis showed that the M2 macrophage markers, CD206 and CD163, were upregulated at the 8th week (Figure 3D,E), whereas the M1 macrophage marker, TNFα, was upregulated at the 16th week of the β1-AA-induced hepatic fibrosis (Figure 3F). Here, MRC1 is linked to Hepatic fibrosis.