To evaluate the potential of exogenous Sema3E to enhance the capability to fight against chlamydial infection, we treated WT mice and Sema3E KO mice intranasally with either recombinant Sema3E-Fc or control saline-Fc, two hours before Cm infection and day 1 to day 6 consecutively after infection (Supplementary Figure 1A). The gene discussed is SEMA3E; the disease is chlamydia trachomatis infectious disease.