We studied the effect of NPC-402 on MAPK signaling in B16F10 melanoma cells by Western blotting and found that NPC-402 treatment decreases the expression of phospho-MEK, phospho-ERK (Figure 4J, Figure S2A), and P-AKT (Figure 4K, Figure S2B) significantly in a dose-dependent nature. This evidence concerns the gene AKT1 and melanoma.