WNT3A and neoplasm: Flow cytometry analysis showed that following the enrichment of CICs in WNT3A transfectants, a majority of CICs were present in sorted SW480-FR/SQ tumor cells expressing constitutively high WNT3A activity (Figure 6E), and this WNT3A activity is required for maintenance of the CIC immunophenotype (CD44v6 [high+]) by promoting tumor sphere formation in CICs (Figure 6F).