To further elucidate the possible role for autocrine effects of PN, IL17A and WNT3A on CICs, we investigated the effects of blocking each of these stromal factors on the viability of CICs (measured by Cell Titer-Glo assay) and on the tumorigenic potential of CICs (measured by quantitation of tumor spheres) in the absence of CAFs using a blocking antibody for each of the stromal factors (Figures 4K and Supplementary Figure 1C). Here, IL17A is linked to neoplasm.