Unlike the discovery that Tsp2 deletion inhibited the AKT/PI3K pathway in gastric cancer and melanoma, we found that pCREB, pERK1/2, pMSK1/2, and p-p38alpha were up-regulated, pAkt1/2/3 and pSTAT1/3 were down-regulated in 94T OE-Tsp2 through the protein microarray analysis. This evidence concerns the gene AKT1 and melanoma.