The heatmap plots of DEGs from RNA-seq analysis (KYSE180 Sh-2 vs. KYSE180 Sh-NC, KYSE410 Sh-2 vs. KYSE410 Sh-NC, |fold change| ≥ 2) were shown in Fig. 4C and Supplementary Fig. S3A. Moreover, pathway analyses showed that TMTC3 expression level was closely associated with cell adhesion in ESCC (Fig. 4D and Supplementary Fig. S3B). Here, TMTC3 is linked to esophageal squamous cell carcinoma.