The methylation-sensitive TFs in these triplets included several critical regulators in AD, such as the estrogen receptor (ESR1)44 and the glucocorticoid receptor (NR3C1)45, as well as factors in biological pathways previously shown to be important in AD pathogenesis, such as antioxidant response (MAFF)46,47, TGFβ signaling (SMAD1)42, Wnt signaling (TCF7L2)48, and hypoxia (H1F1A)49. The gene discussed is SMAD1; the disease is Alzheimer disease.