In this study, upon stringent genotyping and phenotyping, including genotyping at both DNA and protein levels, X‐ray irradiation, off‐target analysis, LOH analysis, and histology‐based nevus/melanoma staging with RT‐PCR validation, we have provided several lines of evidence demonstrating the establishment of a canonical full‐length P53‐deficient X. tropicalis line that develops nevi and melanomas in addition to recapitulating some of the LFS cancers. Here, TP53 is linked to melanoma.