We demonstrate that melanoma cells altered astrocyte secretome and evoked MCP-1 expression and secretion, which in turn induced CCR2 expression in melanoma cells, enhancing the in vitro tumorigenesis (proliferation, migration, and invasion) of melanoma cells, while the inhibition of MCP-1 or CCR2 (via bindarit or CRISPR/Cas9 system) rescued this phenotype in vitro and in vivo. This evidence concerns the gene CCL2 and melanoma.