Nevertheless, coculturing astrocytes with B16-F10, B2905, D4M.3A, and Mel-ret murine melanoma cells or WM115 and 131/4-5B1 human melanoma cells led to 2.1-, 1.1-, 1.5- and 4-fold increase or 15-fold and 15-fold increase, respectively, in their MCP-1 mRNA expression compared with astrocytes grown as monoculture (Figure 2A). The gene discussed is CCL2; the disease is melanoma.