Recombinant human MCP-1 (rh-MCP-1) enhanced melanoma cell migration in a wound healing assay (5% increase in WM115, 27% increase in 131/4-5B1), whereas MCP-1 neutralizing antibody reduced wound closure (7% reduction in WM115, 66% reduction in 131/5B1) compared with cells that migrated in astrocyte SFM or astrocytes CM (Supplemental Figure 7C). This evidence concerns the gene CCL2 and melanoma.