Hence, with the intention of blocking the interaction of cancer cells expressing CCR2 (and CCR4) with MCP-1 secreted in MBM (from astrocytes and other sources of cells in the brain, including tumor cells), we knocked out CCR2 and CCR4 genes in melanoma cells, using the CRISPR/Cas9 system. The gene discussed is CCR2; the disease is neoplasm.