A further independent study applied ChIP-seq analysis to discover Egr1 target genes in the brain of an AD mouse model (APP23), and identified and validated genes directly implicated in AD as Egr1 targets (such as Picalm, Psen2, and App), as well as genes associated with synaptic plasticity and protein transport (such as Arc, Grin1, Syn2, Vamp2, and Stx6) [100]. This evidence concerns the gene PICALM and Alzheimer disease.