Immunohistochemistry and immunofluorescence for phospho-tau (AT8), 3R-tau (RD3), 4R-tau (RD4), and AD-specific tau (GT38) was performed on pons, midbrain, hippocampus, basal ganglia, temporal cortex, midfrontal cortex, and occipital cortex (see Supplement for methods). This evidence concerns the gene MAPT and Alzheimer disease.