CD163 and neoplasm: Notably, we performed immunofluorescence staining on tumor tissue chips, and CD8 positive T cells were labeled with pink fluorescence, CD68 positive macrophages were labeled with red fluorescence, CD163 positive macrophages were labeled with green fluorescence, CLEC5A was labeled with rose-red fluorescence, and DAPI was used to counterstain the nucleus (Figure 5A–L).