We analyzed the expression of the NSUN members in tumor tissues, explored their relationship with patient prognosis and clinicopathological features, further analyzed the correlation between NSUN2 and immune infiltration of prostate tissues, and demonstrated that NSUN2 has an important effect on the proliferation and invasion of prostate cancer cells, which is related to the sensitivity of chemotherapy drugs, and NSUN2 is promising as a novel marker for predicting patient prognosis and therapeutic target. The gene discussed is NSUN2; the disease is prostate cancer.