TP53 and central nervous system cancer: Different combinations of these plasmids have been used to generate highly penetrant gliomas using the SB model, including common mutations seen in HGG such as mutant H3.3K27M, ATRX, ACVR1-G328V, and IDH1, along with tumor drivers NRAS and shRNA KO TP53 (shp53) (34–36).