Our explanation of this negative correlation that the downregulation of Znhit1 expression in BC tissues may downregulate Phosphatase and tensin homolog (PTEN) expression along with activation of Phosphoinositide 3-kinase (PI3K)/A serine threonine protein kinase (Akt) signaling pathway20, PI3K/Akt activation can stimulate the Wnt/β-catenin signaling pathway via the Glycogen synthase kinase 3 beta phosphorylation by the phosphorylated Akt1/2, preventing β-catenin destruction complex formation22. Here, GSK3B is linked to breast cancer.