Subsequently, transferase-mediated dUTP nick-end labeling (TUNEL) assay showed that knocking down SP100-AS1 caused more DNA damage under 2 Gy radiation and reduced Ki67 staining, suggesting that SP100-AS1 downregulation inhibited CRC growth speed, especially when exposed to radiation (Fig. 3D). The gene discussed is MKI67; the disease is colorectal carcinoma.