Treatment with 10 μM DFS also significantly inhibited the invasive capacity of GBM TSs, as determined by 3D matrigel invasion assays (Fig. 2C), as well as markedly reducing the expression of invasiveness-associated marker proteins, including Zeb1, N-cadherin, Snail and Twist (Fig. 2D). Here, TWIST1 is linked to glioblastoma.