Thus, the expression of NLRP3 inflammasome-related indicators in animal and cell models was first detected by Western blot analysis and the outcomes (Fig. 4A, B) indicated that ALI mouse lung tissues and LPS-induced cells showed elevated expression levels of NLRP3, pro-IL-1β, and Caspase-1 p20 protein, but Caspase-1 levels did not significantly change. This evidence concerns the gene CASP1 and acute respiratory distress syndrome.