We next engineered PLC/PRF/5 and sk-Hep1 cell lines with LMNA knockout using CRISPR/Cas9 to further explore the role of crotonylation of lamin A in hepatocellular cancer cells (Fig. S2a, b, c), and the LMNA-KO cells were marked as LMNA-/-. The gene discussed is LMNA; the disease is hepatocellular carcinoma.