We observed a peak in microbe loads at 24 h post-infection in all fly lines (see electronic supplementary material, figure S4 and table S3) at this dose, and all fly lines showed sex differences at this timepoint (figure 2; electronic supplementary material, figure S4 and table S3), though by 96 h following oral infection this sex difference was no longer present in flies lacking upd3 or egfr expression (electronic supplementary material, figure S4 and table S3). This evidence concerns the gene EGFR and infection.