Flow cytometry assays to detect apoptosis were performed (Fig. 3F, G; Additional file 4: Fig S3F, 3G) and the results showed that UNC5B interference increased the apoptosis rate of B-ALL cells treated with recombinant netrin-1 compared with the control group with recombinant netrin-1 treatment and prohibited the anti-apoptotic effect of netrin-1. The gene discussed is NTN1; the disease is acute lymphoblastic leukemia.