In the present study, we observed that the phosphorylation of FAK was upregulated in B-ALL cells under the treatment of recombinant netrin-1 and abolished by Unc5b interference, indicating that FAK was the main downstream effector of Netrin-1-Unc5b binding in B-ALL cells. This evidence concerns the gene UNC5B and precursor B-cell acute lymphoblastic leukemia.