For tumor-derived organoids cultured for 2 weeks, majority of the cells expressed SOX9/MKI67 (SOX9+ 41.6%, MKI67+ 77.5%, SOX9+MKI67+ 37.7%), but after long-term culture (2 months), less cells expressed SOX9 or MKI67. The gene discussed is MKI67; the disease is neoplasm.