HMGB1 and Anxiety: To investigate whether HMGB1 upregulation drives anxiety after p-IONX by influencing activities of mPFC pyramidal neurons, anti-HMGB1 mAb was intraperitoneally administered once daily immediately after p-IONX and the electrophysiological characteristics of mPFC pyramidal neurons in layer 2/3 were then evaluated on D7 PO (Fig. 7a and b).