HMGB1 and Anxiety: To investigate whether HMGB1 participates in the maintenance of comorbid anxiety in neuropathic pain, anti-HMGB1 mAb (2 μg) was infused into bilateral mPFC once daily throughout D30 to D37 after p-IONX and PSL, respectively, when anxiety had been established in both groups of mice (Fig. 5a).